Objectives: Prevention of HPV reinfection by HPV vaccination after surgical treatment of patients with high grade intraepithelial lesion HSIL-CIN 2 and Human papilloma virus (HPV) infection. (Open controlled study)

Methods: There were 145 patients with HSIL-CIN 2 investigated. (Pap smear, colposcopy, biopsy, immunohistochemistry P16 +, PCR).

Results: All investigated patients (n=145) with HSIL-CIN 2 were treated by Co2 Laser Conisation and vaporisation. They were offered HPV vaccination. Main control group included 53 patients who agreed to the vaccination. They were treated with HPV vaccination. After surgical procedure and before sexual activity. Study group included 92 unvaccinated patients. Control PAP smear, colposcopy and PCR detection of HPV (type - 6, 11, 16, 18, 31) infection were done after surgical treatment with 3 month intervals during one year.

There were no HPV induced lesions revealed during one year in main control group. In Study group there were found cases of HPV induced lesion. After 3 month: In 5.6% by colposcopy - TZ, acetowhite epithelium. In Pap smear - HPV induced lesion were not revealed. PCR were positive (16, 18). After 6 months: In 7,5% by colposcopy - TZ, acetowhite epithelium, flat condyloma. In Pap smear - LSIL-CIN 1- HPV, PCR were positive (16, 18). After 9 months: In 11,3% by colposcopy - TZ, acetowhite epithelium, fine punctuation. In Pap smear - LSIL-CIN 1- HPV, PCR were positive (16, 18). After 12 months: In 22,6% by colposcopy - TZ, acetowhite epithelium, puation, mosaic. In Pap smear - LSIL-CIN 1- HPV, PCR were positive (16, 18). HPV induced lesion was statistically significant at 6, 9 and 12 months (p<0.05).

Conclusions: Based on our preliminary data we can suppose that HPV vaccination, after laser surgery of intraepithelial lesion, may prevent HPV reinfection.